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KMID : 0357419940240010043
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Korean journal of Virology
1994 Volume.24 No. 1 p.43 ~ p.52
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Propagation of Varicella-Zoster Virus in Human Embryonic Lung Cells using Microcarrier
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Abstract
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A human embryonic lung fibroblast(LuMA) cell line was established for production of a live attenuated varicella-zoster virus vaccine. Using this cell line, we have investigated the possibility of using microcarrier culture system for producing
cell-associated and cell-free varicellazoster virus. We have also investigated the utilization of glucose and amino acids during cell culture and virus propagation in microcarrier culture. It was found that no infectious cell-free viruses appear
in
the
culture medium, and substantial decreases in cell density was observed as cell culture time increase. The cell-free varicella-zoster virus yield in microcarrier culture was much lower than that of the stationary T-flask culture.
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